Bioburden Testing – How To Do Efficient Microbiological Analyses?

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Hygiene plays a key role in food production. Microorganisms such as bacteria, viruses, yeasts and molds may spoil food and could cause a risk to health. Standard monitoring throughout the manufacturing process is therefore vital. We show you what’s important on your hygiene monitoring and current procedures which make determining microbial counts child’s play. The total bacteria Count is among the major indicators in the area of hygiene management. It indicates how many germs are found in a sample. Monitoring the overall bacteria count is essential, because the number of germs should not exceed certain guide worth. These guide values, which can be expressed in CFU colony-forming units per gram or millilitre, be based on different kinds of food. The expert group Food Microbiology and Hygiene of the German Society for Hygiene and Microbiology provides a practical overview of those guide values.

There are several Methods to ascertain the overall bacteria count in food. One alternative is using a counting chamber. This system is used by counting the number of cells under the microscope. But this method is quite inaccurate and error-prone. Another option is the Most probable number procedure. With this method the sample is diluted in many phases, filled into capsules together using a nutrient solution and incubated at a suitable temperature. The number of tubes in which bacteria are present is an indication of the microbial count. This technique is, however, also quite inaccurate and material-intensive. An easier and more Accurate method to determine the microbial count is that the plate method, where a food sample is placed on a culture medium plate. After an appropriate incubation period, you can count the number of colonies which have formed on the culture medium plate.

A variant of this plate bioburden testing, which can be used in Compact Dry, makes the decision of the microbial count really straightforward. This microbiological rapid test allows the reliable on-site analysis of different samples: liquids drinking water, drinks and solid food can be examined as well as makeup and smears of surfaces. And this is the way it works. Open the lid of the petri dish and place the sample material on the plate. Close to the plate and put it upside down in an incubator for a suitable temperature. Following the incubation period, it is simple to count the coloured colonies with the naked eye. Despite its simplicity, The Compact Dry is very efficient and can detect up to 300 colonies per plate.